- ABOUT US
- SUPPORT THE BRI
Core facilities provide expert training, scientific resources and equipment that might not otherwise be affordable or accessible. The BRI offers a range of core facilities to ensure that BRI members have access to the most advanced technologies for neuroscience research. For information on UCLA's wide variety of core facilities, click http://cores.bioscience.ucla.edu/here.
The following core facilities are operated by the BRI:
Carol Moss Spivak Cell Imaging Facility
The Carol Moss Spivak Cell Imaging Facility is a service of the UCLA Brain Research Institute for the implementation of biological confocal and 2-photon laser-scanning microscopy and some associated technologies. The Facility has a new Leica TCS SP MP Inverted Confocal and 2-Photon Laser-Scanning Microscope, a new Leica TCS SP MP Fixed-Stage Upright Confocal and 2-Photon Laser-Scanning Microscope and a Carl Zeiss LSM 310 laser-scanning confocal microscope, PC and Macintosh computers for image processing and a dye sublimation printer and a film graphics recorder for confocal data output. Some basic equipment is available for physiological experiments involving confocal and 2-photon scanning. These include an Axon Instruments Axopatch 200B Patch Amplifier, a Burleigh micromanipulator, and a Hammamatsu video camera for IR-DIC imaging.
The Director of the Carol Moss Spivak Cell Imaging Center is Dr. Matt Schibler;
The following links have been set up to provide some background in microscopy and to help you make the most of your use of the core facilities and the resources in equipment, services and personnel available to assist members of the Brain Research Institute and the UCLA biomedical community in the area of microscopic imaging. We have included practical information about instrumentation and microscopic theory and techniques as a basis for the use of microscopes in the core facilities as well as in your own laboratories.
Microscopic Techniques Laboratory
The Microscopic Techniques Laboratory is located in Room 78-177 Semel Institute. This facility is prepared to give instructions in microscopic techniques and assist you in preparing tissue specimens for light microscopic observation. · Histological procedures available include some immunocytochemistry staining, special stains, paraffin sectioning, slide preparation for in situ hybridization, cryostat sectioning, plastic embedding and sectioning. The laboratory also provides staining setups, a cryostat, microtomes, and a Nikon photomicroscope for use by trained personnel. · The Microscopic Techniques Laboratory Core Supervisor is Marianne Cilluffo, x59848 (310-825-9848), email@example.com. To use the laboratory and its facilities, you must obtain an Investigator Authorization Form from the Microscopic Techniques Laboratory.
The following services are provided by the BRI Microscopic Techniques Core. For prices, questions, or consultation, contact the Core Supervisor, Marianne Cilluffo, firstname.lastname@example.org; 310-825-9848.
Electron Microscopy Core Facility
The Electron Microscopy Core Facility, in Room 63-377 CHS, houses a JEOL 100CX transmission electron microscope. A Reichert Ultracut ultramicrotome is also available for use by trained personnel. (NOTE: The facility does not supply diamond knives; you must use your own).
Services provided are fixation and embedding of specimens, thin sectioning, and use of the electron microscopes (with or without assistance), gold labeling, negative stain, and light microscopy of plastic embedded materials.
The facility offers advice on appropriate preparatory procedures and other technical matters. Training and assistance in the use of the electron microscope is also offered.
For prices, questions, or consultation, contact the Electron Microscopy Services Center Core Supervisor, Marianne Cilluffo, 310-206-8054, email@example.com. Please see her regarding use authorization and billing.