The Carol Moss Spivak
Cell Imaging Facility is currently setup to perform confocal
and 2-photon laser-scanning microscopy for members of the
UCLA biomedical community. The Facility
has a Leica TCS SP MP Inverted Confocal Microscope,
a Leica TCS-SP MP Fixed-Stage Upright Confocal Microscope
and a Carl Zeiss LSM 310 Laser Scanning Confocal
microscope. These microscopes are housed in
the California NanoSystems Institute, B145.
The Leica Microscopes
have three lasers each, an argon, a krypton, and a helium-neon
which are connected to the microscopes by a fiber optic
and produce laser excitation lines at 476 (near blue) 488
(blue), 568 (yellow) and 633 (far red) nm. These microscopes
are also connected via fiber optic to a A Spectra-Physics
Integrated Two-Photon Laser System comprises a Millenia-X-P
solid state laser and a Model 3941-M Tsunami Ti:Sapphire
laser. The Millenia is a high power, diode pumped solid
state laser that can produce >10W continuous power at 532nm.
The Tsunami is a regeneratively mode-locked Ti:Sapphire
laser and is used in the picosecond configuration.
This combination can be tuned to deliver a pulsed tunable
infrared beam anywhere between 780 and 900 nm for two photon
excitation.* The systems are equipped with three reflectance/fluorescence
photomultiplier/ detectors and one transmitted light detector.
Simultaneous use of the 2-photon laser by both microscopes
is possible provided that the users of each microscope setup
can use the same infrared beam wavelength.
The Zeiss instrument
is equipped with two lasers, an external argon ion blue
laser (488/514 nm) for excitation of green fluorophores
such as FITC, and a helium-neon green laser (543 nm) for
excitation of red fluorophores such as rhodamine and Texas
Red. This instrument has two reflectance/fluorescence
photomultiplier/ detectors and one for transmitted light.
also has a Tektronix 440 Phaser dye sublimation printer
for color print output and a Lasergraphics Personal LFR
Plus film graphics recorder for 35 mm slide output.
is also available for setting up physiological experiments
with the confocal and 2-photon scanning microscopes.
These include an Axon Instruments Axopatch 200B Patch Amplifier,
a Burleigh micromanipulator, and a Hammamatsu video camera
for IR-DIC imaging.
Schibler is in charge of the operation and upkeep of the
facility. Please contact him at x31077 (310-983-1077 off campus) or by E-mail
to be trained in the use of the microscope or to assist
you in examining your specimens.
2-Photon laser system produces 10.5 W of 532 nm green light
from the Millenia laser and 7.5 W of INVISIBLE infrared
laser beam radiation and both lasers are Class IV.
To use these lasers, you will be required to sign a use
consent form and agree to abide by the facility rules for
|Contact Dr. Matt Schibler