The
Carol Moss Spivak Cell Imaging Facility is a service of
the UCLA Brain Research Institute for the implementation
of biological confocal and 2-photon laser-scanning microscopy
and some associated technologies. The Facility has a new
Leica TCS SP MP Inverted Confocal and 2-Photon Laser-Scanning
Microscope, a new Leica TCS SP MP Fixed-Stage Upright
Confocal and 2-Photon Laser-Scanning Microscope and a
Carl Zeiss LSM 310 laser-scanning confocal microscope,
PC and Macintosh computers for image processing and a
dye sublimation printer and a film graphics recorder for
confocal data output. Some basic equipment is available
for physiological experiments involving confocal and 2-photon
scanning. These include an Axon Instruments Axopatch 200B
Patch Amplifier, a Burleigh micromanipulator, and a Hammamatsu
video camera for IR-DIC imaging.
The
Director of the Carol Moss Spivak Cell Imaging Center
is Dr. Matt Schibler; 310-983-1077;
alms@cnsi.ucla.edu.
The
following links have been set up to provide some background
in microscopy and to help you make the most of your use
of the core facilities and the resources in equipment,
services and personnel available to assist members of
the Brain Research Institute and the UCLA biomedical community
in the area of microscopic imaging. We have included
practical information about instrumentation and
microscopic theory and techniques as a basis for the use
of microscopes in the core facilities as well as in your
own laboratories.
The
Basics of Light Microscopy
Transmitted Light Microscopy
Fluorescence
Microscopy
Confocal
Microscopy
2-Photon
Laser Scanning Microscopy
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