·
The
Microscopic Techniques Laboratory is located on the first
floor of the Gonda (Goldschmied) Neuroscience and Genetics
Research Center, Room 1346. This facility is prepared to
give instructions in microscopic techniques and assist you
in preparing tissue specimens for light microscopic observation.
·
Histological
procedures available include some immunocytochemistry staining,
special stains, paraffin sectioning, slide preparation for
in situ hybridization, cryostat sectioning, plastic embedding
and sectioning. The laboratory also provides staining setups,
a cryostat, microtomes, and a Nikon photomicroscope for
use by trained personnel.
· The
Microscopic Techniques Laboratory Director is Marianne
Cilluffo,
x59848 (310-825-9848), mariannc@ucla.edu.
To use the laboratory and its facilities, you must obtain
an Investigator Authorization Form from the Microscopic
Techniques Laboratory.
Charges
Immunocytochemistry |
$10.00/hr |
| Immunofluorescence
staining, DAB reaction, single labeling, double
labeling
|
Plastic
embedding for electron microscope and glycol methacrylate
(GMA) preparation |
$8.00/hr
|
Plastic
sectioning (semi-thin sections only) |
$6.00/hr
|
1-25
slides |
$6.00/slide
|
26-50
slides |
$5.00/slide |
51-100
slides |
$4.00/slide
|
| Paraffin
sectioning and slide preparation for in situ hybridization
and immunocytochemistry (rate per project)
|
1-50
slides |
$3.00/slide
|
51-100
slides |
$2.50/slide
|
101-200
slides |
$2.00/slide
|
201
or more |
$1.50/slide
|
| Routine
histological procedures for paraffin and frozen
sections:(e.g., Nissl and hematoxylin/eosin) (rate
per project)
|
1-50
slides |
$4.00/slide
|
51-100
slides |
$3.50/slide
|
101-200
slides |
$3.00/slide
|
201 or more slides |
$2.50/slide |
| Special
staining:
(e.g., Kluver and iron reaction, Trichrome stains)
$6.00/slide
Instrument use
|
Cryostat
|
$10.00/hr
|
Microtomes:
rotary, sledge, and Leica 205S (for plastic sectioning) |
$10.00/hr
|
Nikon
photomicroscope (for brightfield, fluorescence,
photomicroscopy) |
$10.00/hr
|
Facility
use
|
Staining
setup |
$5.00/hr
|
Olympus
dual viewing microscope |
$5.00/hr |
Project
Development |
$25.00 |
|
COVER
STORY