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· The Microscopic Techniques Laboratory is located on the first floor of the Gonda (Goldschmied) Neuroscience and Genetics Research Center, Room 1346. This facility is prepared to give instructions in microscopic techniques and assist you in preparing tissue specimens for light microscopic observation.
· Histological procedures available include some immunocytochemistry staining, special stains, paraffin sectioning, slide preparation for in situ hybridization, cryostat sectioning, plastic embedding and sectioning. The laboratory also provides staining setups, a cryostat, microtomes, and a Nikon photomicroscope for use by trained personnel.
·
The Microscopic Techniques Laboratory Director is Marianne Cilluffo, x59848 (310-825-9848), mariannc@ucla.edu. To use the laboratory and its facilities, you must obtain an Investigator Authorization Form from the Microscopic Techniques Laboratory.
Charges

Immunocytochemistry
$10.00/hr
Immunofluorescence staining, DAB reaction, single labeling, double labeling
Plastic embedding for electron microscope and glycol methacrylate (GMA) preparation
$8.00/hr
Plastic sectioning (semi-thin sections only)
$6.00/hr
1-25 slides
$6.00/slide
26-50 slides
$5.00/slide
51-100 slides
$4.00/slide
Paraffin sectioning and slide preparation for in situ hybridization and immunocytochemistry (rate per project)
1-50 slides
$3.00/slide
51-100 slides
$2.50/slide
101-200 slides
$2.00/slide
201 or more
$1.50/slide
Routine histological procedures for paraffin and frozen sections:(e.g., Nissl and hematoxylin/eosin) (rate per project)
1-50 slides
$4.00/slide
51-100 slides
$3.50/slide
101-200 slides
$3.00/slide
201 or more slides
$2.50/slide
Special staining:
(e.g., Kluver and iron reaction, Trichrome stains) $6.00/slide
Instrument use
Cryostat
$10.00/hr
Microtomes: rotary, sledge, and Leica 205S (for plastic sectioning)
$10.00/hr
Nikon photomicroscope (for brightfield, fluorescence, photomicroscopy)
$10.00/hr
Facility use
Staining setup
$5.00/hr
Olympus dual viewing microscope
$5.00/hr
Project Development
$25.00

 


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